Figure 9
From: A rice class-XIV kinesin enters the nucleus in response to cold
Effect of Leptomycin B, a specific inhibitor of nuclear export on localisation of OsDLK-GFP. (a) Time course of intranuclear signal increment in response to 200 nM LeptomycinB compared to the solvent control (the same volume of 70% methanol, corresponding to a final concentration of 1.37% MetOH in the assay as control). At least a total of 20 cells per time point and sample were collected in each experimental series. The results were tested for significance using Student’s t-test at 95% and 99% confidence level, labled with asterisks. Error bars represent the standard error of triplicate measurements. (b,c) Representative image of an OsDLK-GFP cell after 3 days of treatment with 200 nM Leptomycin B. GFP signal shown in (b), overlay with differential interference contrast shown in (c). Scale bars: 10 μm.
