Figure 3
From: Increased Glutamine Consumption in Cisplatin-Resistant Cells Has a Negative Impact on Cell Growth
Ddp-resistant cells were resistant to oxidative stress, which was related to the availability of Gln. (A) Relative ROS levels in HeLa and HeLa/ddp cells treated with ddp for 24 h. (B) Relative ROS levels in HeLa and HeLa/ddp cells treated with H2O2 for 24 h. (C and D) Relative cell viability and cell numbers for HeLa and HeLa/ddp cells treated with H2O2 for 72 h. (E) Relative clonogenic growth of six cell lines in the presence of H2O2 (20 μM for HeLa and HeLa/ddp, 10 μM for HGC27, HGC27/ddp, AGS and AGS/ddp). (F and G) GSH-ST activity, GSH-Px activity, GR activity, total glutathione and GSH/GSSG ratio in HeLa and HeLa/ddp cells. (H) Relative ROS levels in HeLa and HeLa/ddp cells cultured with corresponding medium for 24 h. The error bars represent the s.d. of triplicate wells of a representative experiment.
