Figure 3
From: Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins
Engineered curli matrix enhances E. coli adhesion to gut mucosa. (A) Anti-Muc-2 detection of mucins after incubation on top of filtered biofilms producing curli-TFF fusions, (B) Congo Red and Thioflavin T staining of goat colon after incubation with engineered curliated E. coli variants. (C) Viscosity vs. shear rate analysis for mucins combined with curliated bacterial cultures. Ctrl indicates cells containing a plasmid with no CsgA encoding gene. (D) Bacterial adhesion to Caco-2 cell monolayers. Ordinary one way ANOVA with Dunnett’s multiple comparison test n = 10, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
