Figure 4
From: Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins
Bioactivity of displayed trefoil factors on the curli matrix (A). Percent cell monolayer defect closure for Caco-2 cell monolayers during incubation with various soluble semi-purified curli variants. Bars represent mean % defect closure after 24 hours. Ctrl indicates scratched Caco-2 monolayers with no treatment. (B) Representative images of defects and their progressive closure after 24 hours in the presence of various curli fiber variants. (C) Corresponding cell migration assay with soluble trefoil factors. Ordinary one way Anova n = 10, **P < 0.01, ***P < 0.001 Ordinary one way ANOVA with Dunnett’s multiple comparison test n = 10, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
