Figure 7
ER stress inhibitors alleviate cisplatin-induced HBV reactivation in HBV transgenic mouse model. HBV transgenic mice were randomly divided into four groups (n = 6 for each group). Animals received 100 mg/kg PBA or 10 mg/kg 4 μ8C for 1 day, followed by treatment with PBA or 4 μ8C combined with 6.0 mg/kg cisplatin daily for 5 days. On the sixth day, the mice were sacrificed and serum and tissue samples were collected. (a to b) HBV DNA and 3.5 kb mRNA in the liver were detected by real-time PCR. (c) HBV replication intermediates in the liver were detected by Southern blotting. (d) The expression of the indicated ER stress inducible proteins, HBcAg and PGC1α were analyzed by western blot. The cropped blots are used in the figure and full length blots are presented in Supplementary Fig. S11. (e) Liver tissue sections were H&E stained or stained with anti-HBc antibodies (magnification, ×200). (f) A schematic model by which cisplatin induces PGC1α through ER stress and strengthens HBV transcription.
