Figure 3

Chaperone assay of GhHSP proteins. (A) Effect of control protein BSA and inhibitor Nov on activity of Citrate synthase (CS) (B) Effect of control protein BSA and Pif on activity of CS. (C) Effect of Human HSP90 on activity of CS, with or without Nov. (D) Effect of Human HSP70 on activity of CS, with or without Pif. (E) Effect of GhHSP90 on activity of CS, with or without Nov. (F) Effect of GhHSP70 on activity of CS, with or without Pif. (G) The mean CS activity at the end point of A, C and E relative to CS-thermosprotection in the presence of HSP90 protein. (H) The mean CS activity at the end point of B, D and F relative to CS-thermosprotection in the presence of HSP70 protein. The asterisks represent statistical significance between two independent experiments (*p-value ≤ 0.05, **p-value ≤ 0.01).