Table 1 Primers used in the present study.
Target | Primer designation | Sequence (5′-3′) | Target coordinates* | Orientation |
|---|---|---|---|---|
ASFV-I215L | 215FwE | AGACACCTGATAGAGAACCC | 157562–157581 | Forward |
ASFV-I215L | 215FwI | TCCAATGTTCCACCAATACCC | 157069–157089 | Forward |
ASFV-I215L | 215RvI | TCATCCATCTCTTCATCCTCCTC | 156971–156993 | Reverse |
Cyclophilin A | CycloFw1 | AGACAAGGTTCCAAAGACAGCAG | — | Forward |
Cyclophilin A | CycloRev | AGACTGAGTGGTTGGATGGCA | — | Reverse |
Cyclophilin A | CycloFw2 | TGCCATCCAACCACTCAGTCT | — | Forward |
VP72 | VP72Fw | ACGGCGCCCTCTAAAGGT | 88273–88290 | Forward |
VP72 | VP72Rev | CATGGTCAGCTTCAAACGTTTC | 88322–88343 | Reverse |
VP32 | VP32Rev | TCTTTTGTGCAAGCATATACAGCTT | 108162–108186 | Forward |
VP32 | VP32Fw | TGCACATCCTCCTTTGAAACAT | 108228–108249 | Reverse |
ASFV-I215L | 215Fw | ACTAGCTAGCATGGTTTCCAGGTTTTTAATAGCAGAG | 157562–157581 | Forward |
ASFV-I215L | 215Rv | TCCGCTCGAGCTCATCATCCATCTCTTCATCCTC | 157069–157089 | Reverse |
ASFV-I215L | C85AFW | TATTTACCCTGATGGAAGACTAGCTATCTCTATCTTACACGGAGAC | 157336–157381 | Forward |
ASFV-I215L | C85ARV | GTCTCCGTGTAAGATAGAGATAGCTAGTCTTCCATCAGGGTAAATA | 157336–157381 | Reverse |
ASFV-I215L | C162AFW | ATTTTTAAAATATTCTATGTCTTCTGGTGAAGCCTCATCTAATGATTTTTTGACAGTCTTTTTA | 157336–157381 | Forward |
ASFV-I215L | C162ARV | TAAAAAGACTGTCAAAAAATCATTAGATGAGGCTTCACCAGAAGACATAGAATATTTTAAAAAT | 157096–157159 | Reverse |
ASFV-I215L | C189AFW | ATACCCAGTGATGCTTATGAAGATGAAGCTGAAGAAATGGAGGATG | 157029–157029 | Forward |
ASFV-I215L | C189ARV | CATCCTCCATTTCTTCAGCTTCATCTTCATAAGCATCACTGGGTAT | 157029–157029 | Reverse |
