Figure 4
From: Foxm1 controls a pro-stemness microRNA network in neural stem cells
Foxm1 controls the transcription in P4 murine cerebellar NSCs of multiple miRNAs and miRNA clusters. (A) Heat map and dendrogram depiction of the 80 miRNAs displaying significant differential expression in NSCs before and after induction of differentiation. (B,C) qPCR-ChIP assays of NSCs and Diff-NSCs using anti-Foxm1 antibody and anti-acetyl-H3 antibody. Immunoprecipitation with IgG was performed as control. Eluted DNA was PCR-amplified with primers annealing to promoter regions of the miRNA genes of interest. Findings for miRNA candidates belonging to a cluster are based on assays of one representative cluster member. Results are expressed as fold induction versus input controls. B-actin was utilized as unrelated chromatin control and is presented in Supplementary Figure 6B. Bars represent the mean (SD) of three independent experiments. P values NSCs vs. Diff-NSCs (Mann–Whitney U test): Statistically significant (B) Foxm1: **P < 0.01: 0.002204 (miR-17~92); ***P < 0.001: 0.000 (miR-15b~16-2), 0.0003471 (miR-130b), 0.00004 (miR-15a~16-1), 0.0003906 (miR-301a). AcH3: *P < 0.05: 0.049416827 (miR-15b~16-2); **P < 0.01: 0.008868 (miR-130b); ***P < 0.001: 0.0008656 (miR-17~92), 0.00000069 (miR-15a~16-1), 0.00000920 (miR-301a). (C) Foxm1: *P < 0.05: 0.01467 (miR-335), 0.01021 (miR-106b~25); NS: not significant: 0.07214 (miR-130a). AcH3: *P < 0.05: 0.02903 (miR-130a); NS: 0.5259 (miR-335), 0.4417 (miR-106b~25).
