Figure 2 | Scientific Reports

Figure 2

From: Global Gene Expression Analysis in an in vitro Fibroblast Model of Idiopathic Pulmonary Fibrosis Reveals Potential Role for CXCL14/CXCR4

Figure 2The alternative text for this image may have been generated using AI.

Heat Maps for Significant Gene Expression changes through culture in IPF-F and Normal-F. Hierarchical clustering of significant gene expression changes that occur because of culturing IPF-F and normal-F in vitro. Samples are in columns, genes in rows. Those samples denoted in red are fibroblasts that were cultured for three weeks prior to the isolation of RNA, those in black were non-cultured (P0). (A) IPF-F lines at P0 (n = 8) and the corresponding P3 (n = 8) were analyzed. (B) normal-F lines at P0 (n = 4) and the corresponding P3 (n = 4) were similarly analyzed. (C) PCA analysis of the IPF-F demonstrates two clusters corresponding to each time point P0 and P3. (D) PCA analysis of the Normal-F demonstrates a similar pattern of clustering based on P0 or P3 grouping. These samples fall distinctly into two clusters P0 and P3, which represent the significant and global changes that occur as the cell populations adapt to the in vitro environment. The complete gene lists with log ratios is available in Tables S1 and S2.

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