Figure 2 | Scientific Reports

Figure 2

From: An all-trans-retinal-binding opsin peropsin as a potential dark-active and light-inactivated G protein-coupled receptor

Figure 2

Bioluminescent assays for light-dependent changes in intracellular Ca2+ and cAMP levels. (a) Aequorin Ca2+ assay with HEK293 cells heterologously expressing human Opn4, spider peropsin, or no opsin. The inset shows the same data of spider peropsin and no opsin on a linear scale. Averaged values for three samples are shown with error bars (s.e.m.). (b) GloSensor cAMP assay with HEK293 cells expressing jellyfish opsin, spider peropsin, sPeropsin-GsOpL3, or sPeropsin-β2ARL3. (c) GloSensor cAMP assay with HEK293 cells expressing MosOpn3 or sPeropsin-GiOpL3. The same data of spider peropsin shown in Fig. 2b is shown here for comparison. Cells were illuminated with white light (a; arrows) or green light (b,c; arrowheads).

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