Figure 6

The deaminase activity assay on C and mC of A3H and various mutants on loop 1 and loop 7. (A–D) Representative gels of the deaminase assay of the A3H loop 1 and loop 7 mutants, including point mutations on loop 1 (panels A,B), on loop 7 (panels C), and loop swapped mutants of loop 1 and loop 7 (panel D), using cell lysates of transfected HEK293T cells. The deaminase reaction was performed with cell lysate range of 0–6 μg (total protein amount, 2-fold dilutions from 6 μg) and 300 nM ssDNA. (E) A bar graph representation of the quantification of the deaminase assay results from (A–C) (also see Supplementary Table S1).