Table 3 Subcellular distribution of A3H positively charged patch mutants.

From: Understanding the Structure, Multimerization, Subcellular Localization and mC Selectivity of a Genomic Mutator and Anti-HIV Factor APOBEC3H

A3H protein

Nuclear localization

Nuclear/(Nuclear + Cytosol)

Hap I

0.71 ± 0.05

Hap II

0.50 ± 0.14

Hap II m1+W115A

0.77 ± 0.03

A3H hap II mutants

Patch 1

(K16/R17/R18/R20/R21 to A)

0.80 ± 0.03

Patch 2

(K27/K50/K51/K52 to A)

0.65 ± 0.07

Patch 3

(K168/R171/R175/R179 to A)

0.80 ± 0.10

Patch 1a

(K16/R17/R18 to A)

0.68 ± 0.07

Patch 1b

(R20/R21 to A)

0.51 ± 0.02

Patch 3a

(K168/R171 to A)

0.53 ± 0.003

Patch 3b

(R175/R179 to A)

0.68 ± 0.11

  1. The subcellular distribution of different mutants and WT A3Hs was estimated based on the Western blot results of cell fractionation. The cell fractionation results showed that the distribution of the α-tubulin was detected in the cytosol and the histones in the nuclear fraction on the Western blots (Fig. 4B, and Supplementary Figure S6), indicating a clean separation of cytosol and nuclear fractions. S.D. was estimated from data collected in two to three independent experiments.
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