Figure 2

Luminescence signal reports responses to food availability and quality. (a) Mean bioluminescence signal from single LUC:GFP transgenic animals at young-adult stage (YA). Worms were fed on OP50, HB101, or HT115, as indicated. The graph shows data from two experimental replicates. (b) Description of the experiment of fasting and refeeding. YA animals are transferred from an NGM plate with OP50 to a well of a 96-well plate with 10 g/l OP50 in S-basal (Feeding). Alternatively, YA animals starved for 1 hour, are transferred to a well of a 96-well plate with S-basal (Fasting). After two hours of continuous measurement, the wells containing fasting animals are supplemented with either OP50 in S-basal (Refeeding) or S-basal alone, and are measured for another two hours. (c) Mean luminescence signal of two hours measurements for each condition. The graph shows data from one representative experiment of two independent replicates. The replicate is shown in Supplementary Fig. S3b. Error bars show s.d. All experiments were done using a strain carrying the integrated transgene sevIs1.