Figure 3

Luminescence signal respectively increases and decreases upon serotonin and naloxone treatment. (a) Mean pharyngeal pumping per minute (ppm) of single LUC::GFP transgenic animals, at the young-adult stage (YA), in the absence and presence of serotonin (5-HT). The graph shows data from two experimental replicates. (b) Mean luminescence signal from individual LUC::GFP transgenic animals, at the YA stage, in the absence and presence of serotonin (5-HT). The graph shows data from six experimental replicates. (c) Total fluorescence intensity from individual YA LUC::GFP transgenic animals in the absence and presence of serotonin (5-HT). The graph shows data from three experimental replicates. (d) Mean pharyngeal pumping per minute (ppm) of single LUC::GFP transgenic animals, in the absence and presence of naloxone (NLX), at the YA stage. The graph shows data from two independent biological replicates. (e) Mean luminescence signal from individual YA LUC::GFP transgenic animals in the absence or presence of naloxone (NLX). The graph shows data from six experimental replicates. (f) Total fluorescence intensity from individual LUC::GFP transgenic animals, in the absence or presence of naloxone (NLX), at the YA stage. The graph shows data from one representative experiment of two independent replicates. Error bars show s.d. All experiments were done using a strain carrying the integrated transgene sevIs1.