Figure 4

Luminescence signal reports the response to drugs on real time. (a) Kinetics of the luminescence signal before and after serotonin (5-HT) addition. A single LUC::GFP transgenic animal at the young-adult (YA) stage was measured. Red arrow points out the time point of 5-HT addition. (b) Mean luminescence signal before (1) and after (2) 5-HT addition, in single LUC::GFP transgenic animals, at the YA stage. The graph shows mean values over a 2 minutes period (grey shadows (1) and (2) shown in a) from three experimental replicates. (c) Kinetics of the luminescence signal before and after 5-HT addition, in a population of 20 LUC::GFP transgenic animals, at YA, per well. Red arrow indicates the time point of 5-HT addition. (d) Mean luminescence signal before (1) and after (2) 5-HT addition in a population of 20 LUC::GFP transgenic animals, at YA, per well. The graph shows data from two experimental replicates. Error bars show s.d. All experiments were done using a strain carrying the integrated transgene sevIs1.