Figure 1
HIF1α-dependent glycolysis is associated with pro-inflammatory macrophage differentiation during inflammation. Sorted peritoneal exudate macrophages (PEMs) from C57BL/6 mice were stimulated with IL-4 (1000 U), LPS (100 ng/mL), LPS + IFNγ (100 ng/mL) or curdlan (100 ng/mL) for 10–12 h, and the glycolytic pathway activity was determined by the generation of 3H-labeled H2O from [3-3H]-glucose (A). HIF1α protein expression was determined using immunoblot (B). Sorted PEMs from wild-type (WT) C57BL/6 mice or Hif1αflox/flox; Lyz-Cre (HIF1α−/−) mice were stimulated with the indicated stimuli for 10–12 h, and the glycolytic pathway activity was determined (C,D). Data is presented as the means ± SD (n = 4). One representative experiment of three to four independent experiments is shown. *P < 0.05 and ***P < 0.001, compared with the indicated groups.
