Figure 5
Glycolytic pathway activity was required for pro-inflammatory macrophage differentiation. PEMs with indicated treatments for 10–12 h (2-DG, 1 mmol/L; LPS, 100 ng/mL; curdlan, 100 ng/mL; A–D). A. HIF1α mRNA expression of PEMs was determined with qPCR (Value of control groups was set to 1). (B and C) Supernatants were collected, and the indicated cytokine concentration was determined using an ELISA. (D) The glycolytic pathway activity was summarized. Mice were i.p. injected with 1 × 105 of C. albicans yeast and also injected intraperitoneally with 2-DG (2 g/kg body weight) or solvent alone (PBS; Ctrl) for 9 days. 2-DG or PBS were given daily up until the day before the mice were euthanized (E–H). (E) Mouse liver and kidneys were collected, and the CFU was evaluated. (F) Serum TNFα concentration (F). (G) TNFα expression of PEMs with FCM. A representative image is shown in the left figure and data are summarized in the right figure. (H) The glycolytic pathway activity of PEMs. Data are presented as the means ± SD (n = 3–4). One representative experiment of three independent experiments is shown. P < 0.001, compared with the indicated groups.
