Figure 1
Hypoxia promotes exosome secretion in PCa cells. (A) Each cell line was plated (1 × 106 cells in T75 flask) in complete media; and then cultured for 48 hrs in exosome-depleted media under normoxic (21% O2) or hypoxic (1% O2) conditions. Thereafter, conditioned media was collected and exosomes isolated by ultracentrifugation method as detailed in the methods. Exosome concentration and size distribution was measured by NTA. In each case, exosome concentration was normalized with corresponding cell numbers and presented as fold change (normoxia versus hypoxia) for all cell lines. Data represent mean ± SE of five videos. ****p < 0.0001. (B) Representative particle distributions for normoxic and hypoxic exosomes from all cell lines are presented.
