Figure 6
GW4869 reduces clonogenicity of PCa cells. (A–C) E006AA-hT, 22Rv1, and PC3 cells (1,000 cells per well) were cultured overnight in 6-well plates, and then treated once with DMSO (control) or GW4869 (10–20 μM) and cultured under normoxic (21% O2) or hypoxic (1% O2) conditions for 7-8 days. At the end, cells were processed and colony number was counted as detailed in methods. Representative images are presented and data is presented as mean ± SE (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
