Figure 6

Trans-splicing occurrence by exons position in gene bodies and exon and intron lengths. (A) Expression levels of exons according to their position in transcripts (estimated from the RNA-Seq public dataset). (B) Frequency of trans-splicing according to exon position (estimated from SL Trapping). For A and B groups of genes were divided according to their number of annotated exons. Only genes with three, four, five and ten exons are shown. Analysis of variance (ANOVA) were performed followed by pairwise t-test (*p < = 0.05, **p < = 0.01,***p < = 0.001, ****p < = 0.0001). For a better visualization, pairwise differences were also tested by Tukey’s Honest significant difference test and the results are plotted on Figure S4. (C) Distribution of exon lengths in trans-spliced (pink boxes) and non-trans-spliced (blue boxes) genes that present three, four, five and ten exons. (D) Distribution of introns lengths immediately upstream of the splicing event in trans-spliced (pink boxes) and non-trans-spliced (blue boxes) genes presenting three, four, five and ten exons. For data in C and D, t-test were performed: *p < = 0.05, **p < = 0.01,***p < = 0.001, ****p < = 0.0001.