Table 2 RT-LAMP Primer Sequences used to detect ZIKV, human 18 s rRNA, and Ae. aegypti actin.
Target | Primer | Sequence (5′-3′) |
|---|---|---|
Homo sapien 18 S rRNA | 18 SrRNA-L3 | GTTCAAAGCAGGCCCGAG |
18 SrRNA-B3 | CCTCCGACTTTCGTTCTTGA | |
18SrRNA-FIP | TGGCCTCAGTTCCGAAAACCAACCTGGATACCGCAGCTAGG | |
18SrRNA-BIP | GGCATTCGTATTGCGCCGCTGGCAAATGCTTTCGCTCTG | |
18SrRNA-LF | AGAACCGCGGTCCTATTCCATTATT | |
18SrRNA-LB | ATTCCTTGGACCGGCGCAAG | |
ZIKV | Zika-L3 | GCAGAGCAATGGATGGGATA |
Zika-B3 | CCCATCCTTGAGGTACAGCT | |
Zika-FIP | AACCTGAGGGCATGTGCAAACCGCGGTCAGTGGAGATGACT | |
Zika-BIP | CACAGGAGTGGAAACCCTCGACTGAAGTGGTGGGAGCAGAA | |
Zika-LF | TCGATTGGCTTCACAACGC | |
Zika-LB | GGAGCAATTGGGAAGAAGTCC | |
Ae. aegypti actin | Ae21-F3 | CGGCGCCACCACAAGA |
Ae21-B3 | TCGTGCCTGTGTTTGTCG | |
Ae21-LF | ACCGCAAGGCCAAGAACCG | |
Ae21-FIP | TGCTTGGTCCCTGCCTGGGAGACAGCCCACCAGAACGA | |
Ae21-BIP | GAGACGAGAACGGCCCAGCGGGTCTGGTGTGTGTCTTTG |
