Figure 1 | Scientific Reports

Figure 1

From: A Highly Sensitive Detection System based on Proximity-dependent Hybridization with Computer-aided Affinity Maturation of a scFv Antibody

Figure 1

Construction and expression of anti-preS1 scFvD8 and scFvD8-M. (A) Total RNA from hybridoma. Lanes: M: marker 2000; 1 and 2: total RNA from mAb D8 hybridoma. (B) PCR amplification of VH and VL with 87 pairs of primers. (C) Enlarged inset from picture B showing the amplification of VL. (D) PCR amplification of scFv gene. Lanes: M: marker 2000; 1 and 2: the scFv fragment of anti-preS1 D8 assembled with a (G4S1)4 linker. (E) SDS-PAGE identification of the purified protein, which was expressed with the pET28a-SUMO vector in the BL21 host. (F) SDS-PAGE identification of the purified protein, which was expressed with the pcDNA3.4 vector in CHO-S cells.

Back to article page