Figure 6

MAIT cells can detect and kill myeloma cell lines pulsed with 5-OP-RU. (A) (i) Example flow cytometric pseudocolour plots showing MR1-5-OP-RU tetramer staining before and after in vitro expansion of MAIT cells. Plots are gated on total αβ T cells. (ii) Box plots showing the proportion of MAIT cells in PBMCs directly ex-vivo (Pre) and after in vitro MAIT cell expansion (Post) from 3 individual donors as gated in A. (B) Box plots showing percentage cell death (7-AAD⁺ MM cells) of RPMI-8226 or U266 myeloma cell lines after overnight co-culture with sort purified in vitro expanded MAIT or conventional T cells at a 1:2.5 ratio from two donors. Data is representative of 3 independent experiments. Errors bars represent SEM of triplicate wells. (C) Box plots showing cytokine levels in culture supernatant from B. at the end of culture. Cytokine levels were divided by the number of thousand cells per well. Experiment was repeated 3 times each, on cells from 2 healthy donors in triplicate. Representative data is shown. Errors bars represent SEM of triplicate wells.