Figure 2 | Scientific Reports

Figure 2

From: Influenza A viruses alter the stability and antiviral contribution of host E3-ubiquitin ligase Mdm2 during the time-course of infection

Figure 2

IAV alters Mdm2 protein level and its nucleo-cytoplasmic localization during the time-course of infection. Immunofluorescence staining of Mdm2 (red) and influenza NS1 (green) in mock-infected (panels a to h) or infected with H3N2 virus at a MOI of 1 (panels i to x) was performed at different times, as indicated. Nuclei were counterstained with DAPI (blue, panels c,g,k,o,s,w). Merged fluorescent signals are presented in panels d,h,i,p,t and x. Cell details are enlarged (inset). White scale bar = 10 μm. The presented results obtained by confocal immunofluorescence microscopy are representative fields, from several repeated experiments. The relative mean nuclear intensity of NS1 and Mdm2 stainings was measured at 8 and 24 hpi with ImageJ (version 1.51 h - http://imagej.nih.gov/ij), using DAPI staining to define nuclear areas for measurements. Data collected for NS1 and Mdm2 were subject to correlation analysis.

Back to article page