Figure 7

Amounts of cleaved RNA after treatment with RNase H for 1 h. The mixture of 4 μM of the gapmer and 4 or 12 μM of the complementary RNA was treated with 40 U/mL of RNase H in 10 mM Tris buffer containing 100 mM NaCl and 0.5 mM of MgCl₂ at pH 7.2 and 37 °C. Student t test was used to statistical significance of the results. n = 3. (a) 4 μM of the gapmer and 4 μM of the complementary RNA.; SD = 0.8% (HDO only), 3.2% (+ODAGal4), 1.7% (+ODGGal3), p = 0.293 (HDO only/+ODAGal4), 0.204 (HDO only/+ODGGal3). (b) 4 μM of the gapmer and 12 μM of the complementary RNA.; SD = 8.7% (HDO only), 1.2% (+ODAGal4), 3.8% (+ODGGal3), p = 0.343 (HDO only/+ODAGal4), 0.597 (HDO only/+ODGGal3).