Figure 4
From: Solution structure and dynamics of anti-CRISPR AcrIIA4, the Cas9 inhibitor
ITC for the interaction (A) between AcrIIA4 and SpyCas9, and (B) between AcrIIA4 and sgRNA-bound SpyCas9. Raw ITC data (Top panel) and integrated heats of injection (bottom panel) are presented for the titration between AcrIIA4 and SpyCas9. In the bottom panel, the experimental data are shown as solid squares and the least squares best-fit curves derived from a simple one-site binding model are shown as a black line. The thermodynamic parameters for the complex formation are listed in Table 2.
