Figure 7

(a) The results of RNA-seq of fibrosis-associated genes including COL4A1, TIMP2 and MMP14 in HK2 (left column), RPTEC (middle column) and in vivo samples (right column). (b) Immunohistochemistry of collagen type 4 staining for I/R injured mice of both groups. The quantitative analysis is shown for each group (vehicle; N = 6, DZNep; N = 5). (c) Immunohistochemistry of MMP14 staining for I/R injured mice of both groups. The quantitative analysis of MMP14-positive cell counts is shown for each group (vehicle; N = 5, DZNep; N = 6). (d) Validation of RNA-seq using qRT-PCR for TIMP2.