Figure 1 | Scientific Reports

Figure 1

From: SESN2 negatively regulates cell proliferation and casein synthesis by inhibition the amino acid-mediated mTORC1 pathway in cow mammary epithelial cells

Figure 1

SESN2 is negatively regulated by AA in the process of AA-mediated regulation of cell proliferation and casein synthesis in CMEC. (A) Cell proliferation of CMEC treated with EAA or AA was analyzed by MTT assay, the data of “AA− group” was defined as “1”; (B) Cell cycle of CMEC treated with EAA or AA was analyzed by FC; (C) Expression of casein in CMEC treated with EAA or AA was analyzed by WB, the ratio value of “AA− group” was defined as “1”; (D) Expression of proteins related mTORC1 pathway, SESN2 and GCN2, and the phosphorylation ratio of mTOR and S6K1 in CMEC treated with EAA or AA was analyzed by WB, the ratio value of “AA− group” was defined as “1”; (E) Subcellular localization of mTOR in CMEC treated with EAA or AA for 6 h was analyzed by IF. Each bar represents the LSM ± SEM. In the bar charts, different superscript lowercase letters indicate significant difference (p < 0.05). Uncropped images for all Western blots can be found in Supplementary Fig. 4.

Back to article page