Figure 5 | Scientific Reports

Figure 5

From: SESN2 negatively regulates cell proliferation and casein synthesis by inhibition the amino acid-mediated mTORC1 pathway in cow mammary epithelial cells

Figure 5The alternative text for this image may have been generated using AI.

SESN2 negatively regulates AA-mediated lysosomal localization of mTOR in CMEC. (A) Subcellular localization of mTOR in CMEC treated with si-SESN2 was analyzed by IF; (B) Subcellular localization of mTOR in CMEC treated with SESN2 GO was analyzed by IF; (C) Subcellular localization of mTOR in CMEC treated with AA−, AA−/si-SESN2, EAA+, and AA+ was analyzed by IF; (D) Subcellular localization of mTOR in CMEC treated with AA−, EAA+, AA+, EAA+/SESN2 GO, and AA+/SESN2 GO was analyzed by IF. Each bar represents the LSM ± SEM. In the bar charts, different superscript lowercase letters indicate significant differences (p < 0.05). B: Cells were no transfected; NC: Cells were transfected with negative control siRNA; EV: Cells were transfected with empty vector.

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