Figure 6 | Scientific Reports

Figure 6

From: SESN2 negatively regulates cell proliferation and casein synthesis by inhibition the amino acid-mediated mTORC1 pathway in cow mammary epithelial cells

Figure 6

SESN2 negatively regulates cell proliferation and casein synthesis via mTORC1 pathway in CMEC. (A) Cell proliferation of CMEC treated with si-SESN2, si-mTOR, and si-SESN2/si-mTOR was analyzed by MTT assay, the date of “B group” was defined as “1”; (B) Cell cycle of CMEC treated with si-SESN2, si-mTOR, and si-SESN2/si-mTOR was analyzed by FC; (C) Expression of casein in CMEC treated with si-SESN2, si-mTOR, and si-SESN2/si-mTOR was analyzed by WB, the ratio value of “B group” was defined as “1”. (D) The model of SESN2 negative regulates the AA-mediated cell proliferation and casein synthesis via inhibiting mTORC1 pathway. Each bar represents the LSM ± SEM. In the bar charts, different superscript lowercase letters indicate significant differences (p < 0.05). B: Cells were no transfected. Uncropped images for all Western blots can be found in Supplementary Fig. 4.

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