Figure 3
From: Conditional knockout of NaV1.6 in adult mice ameliorates neuropathic pain
Motor and acute pain behaviors in NaV1.8-Cre driven NaV1.6 KO mice. (a) Motor function: rotarod test (Control, n = 6; NaV1.6Nav1.8 KO, n = 9). (b) Low-threshold mechanical threshold: von Frey test (Control, n = 8; NaV1.6Nav1.8 KO, n = 10). (c) High-threshold mechanical threshold: Randall Sellito test (Control, n = 5; NaV1.6Nav1.8 KO, n = 9). (d) Noxious heat (spinal reflex) threshold: Hargreaves test apparatus (Control, n = 8; NaV1.6Nav1.8 KO, n = 10). (e) Noxious heat thresholds (Supraspinal response) measured using hot plate test at 52 °C and 56 °C (Control, n = 12; NaV1.6Nav1.8 KO, n = 16). (f) Dynamic cold plate test: the number of jumps/paw lifts was measured as temperature of the plate is changed from 25 °C to 0.5 °C (Control, n = 4; NaV1.6Nav1.8 KO, n = 9). (j) Thermal place preference test: the percentage of time spent at a test temperature (20 °C, 28 °C, 38 °C or 46 °C) versus control temperature 33 °C (Control, n = 7; NaV1.6Nav1.8 KO, n = 9). (g) Noxious cold thresholds: cold plate test at 0.5 °C (Control, n = 10; NaV1.6Nav1.8 KO, n = 10). (h) Cooling: dry ice test (Control, n = 5; NaV1.6Nav1.8 KO, n = 9). (i) Cooling: acetone test (Contorl, n = 5; NaV1.6Nav1.8 KO, n = 9). Color code: Control, white; NaV1.6Nav1.8 KO, orange. Data are presented as mean ± SEM. No difference (p > 0.05) was observed based on unpaired t test.
