Figure 7
From: Conditional knockout of NaV1.6 in adult mice ameliorates neuropathic pain
Absence of NaV1.6 decreases excitability of large DRG neurons following SNI. (a) Representative traces from a large DRG neuron expressing AAV-GFP (WT control), showing subthreshold responses to 900–945 pA current injections and subsequent all-or-none action potential by injection of 950 pA (current threshold for this neuron). (b) The same threshold protocol was applied to a large DRG neuron expressing AAV-CRE (NaV1.6AAV KO). The current threshold was 2445 pA for this neuron. Arrows with numbers indicate the current amplitude used to elicit the labeled response. (c) Comparison of current threshold in large WT control (n = 8) and NaV1.6AAV KO (n = 13) DRG neurons. (d) Comparison of current threshold in small WT control (n = 7) and NaV1.6AAV KO (n = 7) DRG neurons. (e) Scatter plot of maximal action potentials in response to external current stimuli up to 2000 pA in large WT control (n = 7) and NaV1.6AAV KO (n = 13) DRG neuron. (f) Scatter plot of maximal action potentials in response to external current stimuli up to 500 pA in small WT control (n = 6) and NaV1.6AAV KO (n = 7) DRG neurons. Data are presented as mean ± SEM. *p < 0.05 based on unpaired t test.
