Figure 6

Effects of a short-term ipragliflozin treatment on renal hypoxia in db/db mice. (a) The design of the experiment. (b) A short-term treatment with high-dose ipragliflozin lowered blood glucose levels in db/db mice. (c) Positive staining for pimonidazole was detected in the outer medulla in db/db mice. (d) Both one- and two-week administration of ipragliflozin reduced positive staining for pimonidazole in the outer cortex, not in the outer medulla. Data show the means ± SD, *p < 0.05 vs db/db (−), ^#p < 0.05 vs db/db-LD, ^†p < 0.05 vs db/db-HD, Bar = 100 μm in a low power field picture and 50 μm in other pictures in (c). n = 4–5 mice in each group, one-way ANOVA, Tukey’s multiple comparison.