Figure 2
Lipoprotein secretion profile of iHLCs and hepatoma cells. (a) Western blot analysis of density gradient fractions with antibodies against ApoE and ApoB. For characterisation of lipoproteins secreted by iHLCs, Huh7, and Huh7.5 cells, cell culture supernatants were subjected to linear iodixanol density gradients. For comparison of lipoprotein distribution, human serum was analysed as well. Shown is one representative experiment. Full-length blots are presented in Supplementary Figures 2 and 3. (b) Distribution of ApoE and ApoB within the gradient. Western blot signals for ApoE and ApoB were quantified using the densitometric quantification function of Fiji. Shown is the relative amount of lipoproteins as per cent of total plotted against the density of the respective fraction (n = 3). All full-length blots used for quantification are presented in Supplementary Figures 2 and 3. (c) Determination of triglycerides in gradient fractions of iHLCs and human serum (mean ± SEM, n = 3).
