Figure 3
Immature and mature iHLCs are permissive to HCV infection. (a) Scheme of the experimental setup. (b) Permissivity at different stages of differentiation. Cells were infected with cell culture derived Jc1p7-GLuc-2A-NS2 from day 12–20 of differentiation and viral replication was monitored for 7 days. 24 h post infection, cells were washed and the last wash was kept as T = 0. Gaussia luciferase activity was measured in the supernatant and in the washes after media change at the indicated time points. The dashed horizontal line represents the background luciferase activity determined in mock-infected iHLCs. (c) Re-infection of Huh7.5 cells with supernatant collected during (b). Huh7.5 cells were infected with the iHLC supernatant from T = 1–5 to determine the production of infectious viral particles at different stages of differentiation. Gaussia luciferase activity was measured 2 days post re-infection (Mean ± SD, duplicate measurements). The dashed horizontal line represents the background luciferase activity determined in mock-infected Huh7.5 cells.
