Figure 2 | Scientific Reports

Figure 2

From: Voxel-wise comparisons of cellular microstructure and diffusion-MRI in mouse hippocampus using 3D Bridging of Optically-clear histology with Neuroimaging Data (3D-BOND)

Figure 2

3D histological data from optically-clear tissue at macro- and microscale. CLARITY-processing combined with immunohistochemistry and confocal microscopy allowed whole brain imaging at cellular resolution. (A) GFAP-positive astrocytes (green), parvalbumin-positive interneurons (red) and tomato lectin-positive blood vessels (white) with DAPI stained cell nuclei (blue) in a cerebral hemisphere from an adult mouse brain. (B) High magnification image of boxed region from (A) shows the resolution of the image. (C and D) Individual channels were separated to show regional differences in cellular distribution, (C) green channel - GFAP positive astrocytes are particularly dense within the hippocampus and white matter, (D) red channel - parvalbumin positive interneurons are predominantly found within the cortex and pyramidal layers of the hippocampaus. Scale bar: A,C and D = 1 mm, B = 100 μm.

Back to article page