Figure 1

GNMT participates in the metabolism of AAI. (a,c) Body weight (BW) of wild-type (WT) C57BL/6 mice intraperitoneally injected with 2 or 5 mg/kg BW/day AAI (AAI 2 or AAI 5) or corn oil (vehicle control), 5 days/week for 3 weeks. Values presented are mean ± SEM, n = 5. p-values were calculated by two-way ANOVA and Sidak’s multiple comparisons test. (b,d) The serum levels of alanine aminotransferase (ALT), creatinine (SCr) and blood urea nitrogen (BUN) were measured from mice at the day before (B) and after (A) the 3-week AAI treatment. The biochemistry parameters from the same mouse before and after AAI-treatment were analyzed by Paired t-test. p-values for the comparison of biochemistry parameters from AAI- and corn oil-treated mice were calculated by Student’s t-test. (e) Histological examination of the kidney and liver tissue in AAI- and corn oil-treated WT mice (hematoxylin and eosin stain, original magnification ×200). (f–i) mRNA levels of Gnmt, Nqo1, Cyp1A1, Cyp1A2 genes in the livers (f, female liver; g, male liver) and kidneys (h, female kidney; i, male kidney) from AAI- or corn oil-treated WT mice were determined by qPCR. Data were normalized to β-actin. All values are presented as the mean ± SEM, n = 5. p-values were calculated by Student’s t-test. *p < 0.05, **p < 0.001, ***p ≤ 0.0001.