Figure 3 | Scientific Reports

Figure 3

From: Glycine N-methyltransferase inhibits aristolochic acid nephropathy by increasing CYP3A44 and decreasing NQO1 expression in female mouse hepatocytes

Figure 3

GNMT prevented AAI-induced kidney injury by regulating cytochrome p450 enzymes in female mouse hepatocytes. RNA microarray analysis of the livers from AAI- or corn oil (vehicle control)-treated WT mice, as described in Fig. 1a and c. (a) The numbers of signature genes in liver in response to AAI treatment. Genes were identified as significantly changed if the fold change was greater than 2 (up or down) and the p-value was less than 0.01 in comparison to the control group. (b) Heat map from hierarchical clustering of xenobiotic metabolism genes. Red and green colors indicate an upregulation and downregulation of gene expression, respectively. Trees on the left of the heat map shows the gene clusters. (c–e) mRNA level of Cyp3A44, Cyp3A41and Cyp2B10 genes in the liver of AAI- or corn oil-treated hGNMT Tg (Tg) and WTL mice, as described in Fig. 2a and b, were determined by qPCR. Data were normalized to β-actin. (C: vehicle control, F: female, M: male). Values are presented as the mean ± SEM, n = 5. p-values were calculated using Student’s t-test. *p < 0.05, **p < 0.001, ***p ≤ 0.0001.

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