Figure 2 | Scientific Reports

Figure 2

From: Analysis of the oligomeric states of nucleophosmin using size exclusion chromatography

Figure 2

Distribution of NPM1 by simple cell fractionation. (A) Summary of the process of cell fractionation. (B) Distribution of NPM1 in the sample prepared in A. (C) Immunofluorescence of endogenous NPM1. (Upper panel) HeLa cells were fixed with formaldehyde, then permeabilized using Triton X-100. (Lower panel) HeLa cells were permeabilized using Triton X-100, then fixed with formaldehyde. Scale bar, 10 μm.

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