Figure 3
From: Analysis of the oligomeric states of nucleophosmin using size exclusion chromatography
Elution profiles of endogenous NPM1 in HeLa cells. (A) (Upper panel) Elution profiles of Triton X-100 soluble NPM1 and RNA. (Lower panel) Elution profiles of Triton X-100 insoluble NPM1 and RNA. Endogenous NPM1 was detected by immunoblot. Protein mass standards are indicated above the panel: thyroglobulin (669 kDa), ferritin (440 kDa), aldolase (158 kDa), conalbumin (75 kDa), ovalbumin (44 kDa), and Ribonuclease A (13.7 kDa). RNA was detected by ethidium bromide staining. The four NPM1 groups (Group 1 to Group 4) identified by SEC are underlined. (B) Schematic representation of sample preparation in C. (C) Elution profile of sonication-treated Triton X-100 soluble NPM1 prepared in B.
