Figure 4
From: Analysis of the oligomeric states of nucleophosmin using size exclusion chromatography
Association of NPM1 with RNA in the high molecular weight fractions. (A) Triton X-100 soluble and insoluble cell lysates were subjected to SEC. The indicated fractions were pooled, then immunoprecipitated with anti-NPM. NPM1 and RNA were detected by immunoblot and ethidium bromide staining, respectively. (B) HeLa cell lysates prepared as in Fig. 2A were treated with or without RNase A, then subjected to SEC. Fractions containing the four NPM1 groups identified in Fig. 3A are underlined.
