Figure 6
VLDL increases CLCF1 binding to CNTFR-expressing cells. Ba/F3 transfected with CNTFRα, gp130 and LIFRβ cDNA, IMR32 neuroblastoma or 3T3-L1 pre-adipocytes were successively incubated with species-matched biotinylated CLCF1 (1 μg/ml) alone or in the presence of LDL, HDL or VLDL (all at 100 μg/ml). Cells were then incubated with PE-conjugated streptavidin and fluorescence was assessed by flow cytometry. Vehicle: cells incubated with PE-streptavidin alone. Histograms indicate the mean fluorescence intensity of 3 independent samples ± SD.
