Table 1 Primers used in this paper.
No. | Primers | Sequence (5′-3′) | Product |
|---|---|---|---|
1 | sopB-for sopB-rev | attcgttaattgcgcgcgtagg gaatattcaggccagttatgct | sopB |
2 | repA-for repA-rev | catggcggaaacagcggttatc atgtatgagaggcgcattggag | repA |
3 | ΔgJ/gJR-for ΔgJ/gJR -rev | tttatattgacgcggaatgtt cgctgagtatttattcatttcct | ΔgJ/gJR identification product |
4 | ΔgJ-Kana-for ΔgJ-Kana-rev | gagtaatttaatgcaagcgatgtaggcctcctgtcgtagtccttatctcatgcagggtgtaggctggagctgcttc aacaacaacagaactgtaatgggtacattaaacatacgcgcatatacatattgccgccatatgaatatcctccttag | Kana gene flanked by homology arms of gJ |
5 | gJR-gJ-for gJR-gJ -rev | agagtaatttaatgcaagcga gaagcagctccagcctacactcataccatacaaaggcat | gJ fragment with left homology arm of gJ |
6 | gJR-Kana-for gJR-Kana-rev | atgcctttgtatggtatgagtgtaggctggagctgcttc aacaacaacagaactgtaatgggtacattaaacatacgcgcatatacatattgccgatgggaattagccatggtcc | Kana fragment with right homology arm of gJ |
