Table 2 Yield of recombinant salmocins expressed in Nicotiana benthamiana plants.

From: Plant-made Salmonella bacteriocins salmocins for control of Salmonella pathovars

No.

Bacteriocin

Harvest (dpi)

Yield (mg/g FW)*

Yield (% TSP)*

AV

SD

N

AV

SD

N

1

SalE2

6

1.7

0.2

3

25.0

0.0

3

2

SalE3

5

1.6

0.2

3

37.0

10.4

3

3

SalE7

5

1.4

0.3

3

18.0

6.9

3

4

SalE1a

5

1.2

0.2

3

20.3

3.1

3

5

SalE1b

4

1.2

0.1

3

25.7

3.1

3

  1. *The yield was calculated in mg/g fresh weight of plant biomass and as a percentage of TSP (based on extraction with 50 mM HEPES pH 7.0, 10 mM K acetate, 5 mM Mg acetate, 10% (v/v) glycerol, 0.05% (v/v) Tween-20, 300 mM NaCl and syringe inoculation of plant material with 1:100 dilutions of agrobacterial cultures (OD600 = 1.3)) and is represented as an average value and standard deviation (AV, SD) of several experiments. N, number of independent experiments. In each experiment, plant material pooled from 3 plants was analyzed for each protein.
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