Figure 2 | Scientific Reports

Figure 2

From: Cisplatin generates oxidative stress which is accompanied by rapid shifts in central carbon metabolism

Figure 2The alternative text for this image may have been generated using AI.

Cisplatin generates oxidative stress in HNSCC. (A) Following CDDP treatment total cell extracts and the DNA-bound cellular fraction were subjected to ICP-MS for quantitative measurement of the CDDP concentration (ppb = parts per billion). (B–D) Cells were exposed to CDDP for 16 hours [2.5 µM], fixed and immunolabeled with phospho-γH2AX antibody. Using high throughput microscopy and single cell image analysis, two parameters were evaluated as a function of CDDP concentration: (C) % positive cells (nuclear intensity > mean +1 standard deviation of negative controls) and (D) number of foci/cell. *Indicates p < 0.05 compared to control condition.

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