Figure 2 | Scientific Reports

Figure 2

From: Cisplatin generates oxidative stress which is accompanied by rapid shifts in central carbon metabolism

Figure 2

Cisplatin generates oxidative stress in HNSCC. (A) Following CDDP treatment total cell extracts and the DNA-bound cellular fraction were subjected to ICP-MS for quantitative measurement of the CDDP concentration (ppb = parts per billion). (B–D) Cells were exposed to CDDP for 16 hours [2.5 µM], fixed and immunolabeled with phospho-γH2AX antibody. Using high throughput microscopy and single cell image analysis, two parameters were evaluated as a function of CDDP concentration: (C) % positive cells (nuclear intensity > mean +1 standard deviation of negative controls) and (D) number of foci/cell. *Indicates p < 0.05 compared to control condition.

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