Figure 5
Cisplatin alters central carbon flux in HNSCC. HN30 cells were exposed to 10 mM all carbon (U) 13C-labeled glucose for 3 hours in the presence or absence of CDDP [10 µM]. Cells were harvested and metabolite levels (unlabeled- m + 0, labeled- m + (2–6)) were quantitatively measured. Each condition was tested in triplicate. Data are presented as means, with error bars indicating standard error of the mean. (R/R/X-5-P = ribose/ribulose/xylulose-5-phosphate, G-6-P = glucose-6-phosphate, F-6-P = fructose-6-phosphate, F-1,6-P = fructose 1,6 bisphosphate); m + X indicates mass shift of X from unlabeled glucose value. *Indicates change is statistically significant compared to the control condition, p-value < 0.05.
