Figure 8
Changes in tumor metabolites correlate with cisplatin anti-tumorigenic effects. (A) Biochemical model linking cisplatin induced oxidative stress with carbon flux. (B) HNSCC cells were exposed to CDDP for 3 hours and intra-cellular lactate levels were measured biochemically; data are expressed as averages, with error bars presented standard deviation and all data are normalized to the control condition. Each condition was tested at least in triplicate. *Indicates p < 0.05. (C,D) HN30 and HN31 dual flank tumors were generated by injecting 2 × 106 cells/tumor. Tumors were allowed to grow for 13 days. Mice were treated with a single dose of cisplatin (5 mg/kg). One flank tumor was harvested at 1 hour after treatment and subjected to metabolic interrogation (B). *Indicates p < 0.05 compared to control condition. The contralateral tumor was allowed to grow until day 30–31 and tumor size measurements were recorded at regular intervals (C).
