Figure 6

SDS-PAGE analysis of aggregated proteins in WT, ∆clpA and ∆clpB strains of S. Typhimurium. Mid-log grown cultures of various strains were exposed to different concentrations of HOCl for 30 min. Aggregated proteins from such cells were prepared and analysed on 10% SDS-gel. Relative quantification was done using ImageJ software(NIH) bundled with 32 bit Java 1.8.0 and expressed as fold difference from protein aggregates isolated from WT strain incubated with 0 mM HOCl. Quantification is indicated below each lane. Experiment was performed two times.