Figure 2
From: Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates
Binding of SLeX-conjugated 30 nm and 100 nm FND-PEG-SA samples to fixed mouse brain endothelial cells. Left: 30 nm FNDs, Right: 100 nm FNDs (a). FND-PEG-SA and the monovalent SLeX-biotin in cells expressing E-selectin (b) FND-PEG-SA and monovalent SLeX-biotin in cells not expressing E-selectin. (c) FND-PEG-SA and multivalent SLeX-PAA-biotin in cells expressing E-selectin (d) FND-PEG-SA and multivalent SLeX-PAA-biotin in cells not expressing E-selectin. (e) Endothelial cells without any nanodiamonds. All images were acquired using the same exposure time of 14 s on the FND channel. An overlay of DAPI (blue) and FND channels (pink) on the bright field image is shown. In each image, the section on each image indicated by a white box is magnified 2.5x.
