Figure 3
From: Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates
Binding of SLeX-conjugated SA-BHHTEGST-Eu samples to fixed mouse brain endothelial cells. Left: DAPI, middle: Eu Red luminescence TGL image, right: overlay (a) SA-BHHTEGST and the monovalent SLeX-biotin in cells expressing E-selectin (b) SA-BHHTEGST and the monovalent SLeX-biotin in cells not expressing E-selectin. (c) SA-BHHTEGST and the multivalent SLeX-PAA-biotin in cells expressing E-selectin (d) SA-BHHTEGST and the multivalent SLeX-PAA-biotin in cells not expressing E-selectin. TGL Images were captured using identical exposure times (5.0 s).
